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As a forensic pathologist,you have just completed an autopsy of a poisoning victim.After a thorough examination,you conclude that the victim died of cyanide poisoning.You know that cyanide binds to the cytochrome oxidase complex,and therefore list the official cause of death as suffocation due to cyanide exposure.However,if you wanted to provide a more technical explanation as to the cause of death,which process was specifically inhibited directly by cyanide?


A) The reduction of NAD+
B) The oxidation of FADH2
C) All proton pumping into the intermembrane space
D) The formation of water from oxygen
E) ATP synthesis

F) B) and D)
G) B) and E)

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What is different about the way that NADH and FADH2 donate electrons to the electron transport chain?


A) NADH is oxidized and FADH2 is reduced.
B) NADH contributes its electrons to the first transmembrane complex in the electron transport chain and FADH2 contributes its electrons after the first transmembrane complex.
C) More protons are transported into the intermembrane space of the mitochondria in response to one molecule of FADH2 as compared to the number of protons transported in response to one molecule of NADH.
D) The electrons from NADH ultimately go on to reduce oxygen to generate water,whereas the electrons from FADH2 are used to reduce pyruvate to lactate.

E) All of the above
F) A) and C)

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You decide to go on vacation in the mountains,where you are staying in a cabin.Unfortunately,when you turn on the water in the cabin you smell hydrogen sulfide (H2S) gas.After some research,you find out that the H2S may be due to the presence of sulfur bacteria living in your pipes.What molecule do these bacteria use as an electron acceptor?


A) O2
B) H20
C) SO4
D) H2S

E) A) and D)
F) All of the above

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Many types of cancer cells have been detected to secrete significant levels of lactate.Do you think these cells are likely undergoing beta-oxidation?


A) Yes,because beta-oxidation can generate intermediates that would lead to the production of lactate.
B) No,because if lactate is being produced,the cell is not likely making use of the pathways needed to make use of the products of beta-oxidation.
C) Yes,because lactate stimulates beta-oxidation.
D) No,because lactate is consumed in beta-oxidation

E) A) and B)
F) B) and C)

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A human cell has a mutation in the gene that encodes the enzyme that generates lactate from pyruvate,rendering that enzyme completely non-functional.Assuming that there is ample glucose present,how would this cell generate energy in the presence of oxygen?


A) Glycolysis coupled with ethanol fermentation
B) Aerobic respiration
C) Primarily through the break down of proteins into amino acids
D) This cell would have no way to generate energy under these conditions because it cannot carry out the reactions needed for glycolysis

E) A) and C)
F) B) and C)

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In aerobic respiration,chemiosmotic generation of ATP is driven by:


A) Pi transfer through the plasma membrane.
B) the Na+/K+ pump.
C) a difference in H+ concentration on the two sides of the inner mitochondrial membrane.
D) osmosis of macromolecules.
E) large quantities of ADP.

F) A) and D)
G) A) and E)

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The equation for cellular respiration is: C6H12O6 + 6O2 →→ 6CO2 + 6H2O.At what specific point in the cellular respiration process has glucose been broken down completely from a six carbon molecule to 6 molecules of CO2?


A) During the priming reactions in glycolysis
B) During the oxidation and ATP formation reactions in glycolysis
C) During pyruvate oxidation
D) During the condensation reaction in the Citric acid cycle
E) During the second oxidation in the Citric acid cycle

F) A) and C)
G) C) and D)

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Organisms that can manufacture their own chemical energy are called ________.


A) autotrophs
B) heterotrophs
C) oligotrophs
D) chemotrophs

E) A) and D)
F) B) and C)

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Cardiac muscle cells need to generate significant amounts of ATP to allow for constant contractile activity.As a result,they primarily depend upon beta-oxidation of fatty acids,which has a higher energy yield than the catabolism of glucose.What would be the ATP yield for beta-oxidation of a hypothetical 10-carbon fatty acid?


A) 32 ATP
B) 35 ATP
C) 50 ATP
D) 62 ATP
E) 65 ATP

F) None of the above
G) B) and C)

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If you take into account the amount of ATP generated by ATP synthase per molecule of NADH produced in aerobic respiration,the net number of ATP molecules produced by substrate-level phosphorylation,and the fact that NADH molecules produced in the cytoplasm have to be transported into the mitochondria,what is the predicted energy yield of glycolysis in eukaryotic cells?


A) 2 ATP
B) 5 ATP
C) 7 ATP
D) 32 ATP
E) 30 ATP

F) None of the above
G) C) and E)

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It is thought that the oldest stage of cellular respiration from an evolutionary perspective is


A) the Citric acid cycle.
B) the electron transport chain.
C) fermentation.
D) glycolysis.

E) C) and D)
F) A) and B)

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In the absence of oxygen,can cells utilize the electron transport chain?


A) Yes,all cells can make use of the electron transport chain in the absence of oxygen via fermentation.
B) No,oxygen is a required cofactor for the complexes in the electron transport chain.
C) Yes,in the case that a cell can use a terminal electron acceptor other than oxygen,it can make use of the electron transport chain.
D) No,oxygen is the primary electron acceptor in electron transport chains in all cell types.

E) B) and C)
F) A) and D)

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As electrons move along the electron transport chain,they lose potential energy.How is the energy that is released used by the cell?


A) The energy is used to transport protons against their concentration gradient
B) The energy is used to pump electrons along the electron transport chain
C) The energy is converted directly into ATP
D) The energy is used to pump NAD+ into the cytoplasm so it can be used in glycolysis

E) All of the above
F) None of the above

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How and where is ATP made in a eukaryotic cell?


A) ATP is only made in the mitochondria in response to chemiosmosis.
B) ATP is made in all compartments of the cell in response to endergonic reactions and is used to drive exergonic reactions in the cell.
C) ATP can be made by direct phosphorylation of ADP in the cytoplasm,and by an enzyme complex that uses the energy from a proton gradient to drive ATP synthesis in the mitochondria.It can also be made in other locations in the cell,depending on the cell type.
D) ATP can be made by an enzyme complex that uses the energy of protons moving down their concentration gradient from the mitochondrial matrix to the cytoplasm to make the ATP.

E) A) and C)
F) A) and D)

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Why are the components of the electron transport chain embedded in the inner mitochondrial membrane rather than floating freely in the cytoplasm of mitochondrial matrix?


A) To generate and maintain the proton gradient essential for ATP production.
B) To separate the ATP from the ADP.
C) Because electrons cannot float in the matrix.
D) Because NADH cannot localize to the mitochondrial matrix.

E) A) and C)
F) A) and B)

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In animals that take in oxygen from their environment,glucose is broken down into carbon dioxide and water in a process called


A) anaerobic respiration.
B) organic compound respiration.
C) glucose respiration.
D) aerobic respiration.

E) A) and B)
F) A) and C)

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A biochemist wants to control the initial substrate-level phosphorylation that occurs in the tracheal cells of grasshoppers once glucose has crossed the plasma membrane.He has access to the following inhibitors: Rotenone - an electron transport chain inhibitor,Oligomycin - an ATP synthase inhibitor,and TLN-232,an inhibitor of glycolysis.Which inhibitor should he use to slow down initial substrate-level phosphorylation that occurs once glucose has crossed the plasma membrane?


A) Rotenone
B) Oligomycin
C) TLN-232
D) None of these inhibitors would be effective in preventing substrate-level phosphorylation

E) B) and C)
F) A) and B)

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What must happen to amino acids before they can be used in catabolic reactions?


A) They must be decarboxylated
B) They must be deoxygenated
C) They must be dehydrogenated
D) They must be deaminated

E) A) and B)
F) All of the above

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What happens to the oxygen that is used in cellular respiration?


A) It is converted to carbon dioxide
B) It is used to make glucose
C) It is used to make Citric acid cycle intermediates
D) It is reduced to form water
E) It is converted to acetyl-CoA

F) A) and E)
G) A) and D)

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When amino acids are degraded in cells,into what intermediate(s) of the aerobic respiration process are the carbon skeletons of amino acids primarily converted?


A) Pyruvate
B) Acetyl-CoA
C) Citric acid cycle intermediates
D) Pyruvate and acetyl-CoA
E) Pyruvate and Citric acid cycle intermediates

F) A) and B)
G) None of the above

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