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cDNA contains introns


A) True
B) False
C) It varies from cDNA to cDNA

D) A) and B)
E) A) and C)

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B

What is the purpose of phosphorylating the carboxy terminal domain (CTD) ?


A) Convert from initiation to the elongation stage
B) To aid in transcriptional initiation
C) Phosphorylate transcription factors
D) More than one above

E) None of the above
F) B) and D)

Correct Answer

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What is the catalytic subunit of prokaryotic RNA polymerase?


A) a
B) b
C) b'
D) σ
E) More than one of the answers

F) B) and E)
G) None of the above

Correct Answer

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How many basal transcription factors are there?


A) 3
B) 4
C) 5
D) 6

E) A) and B)
F) C) and D)

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The RNA polymerase forms a closed complex during the process of elongation during transcription.

A) True
B) False

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Which of the following is not part of the basal transcription apparatus?


A) DNA
B) TFIID
C) RNA polymerase II
D) Mediator
E) TFIIB

F) C) and D)
G) A) and C)

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A functional protein would contain the information contained within which of the following regions of DNA?


A) Exons
B) Introns
C) Enhancers
D) Promoters
E) All of the answers are correct

F) A) and B)
G) A) and C)

Correct Answer

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Alternative splicing allows an organism to .


A) Carry fewer genes
B) Carry more genes
C) Produce fewer gene products
D) More than one of the answers

E) A) and C)
F) A) and B)

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A mutation in which of the following would result in a failure of the RNA polymerase to maintain an open complex?


A) TFIIB
B) TFIID
C) TFIIE
D) TFIIH
E) TFIIF

F) A) and E)
G) A) and D)

Correct Answer

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Which of the following processes is important for the initiation of translation?


A) Alternative splicing
B) RNA editing
C) 5' capping
D) 3' polyA tailing
E) Trimming

F) A) and D)
G) B) and D)

Correct Answer

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Which of the following is true of tRNA processing in


A) RNaseP acts as an endonuclease
B) The recognition sequence of RNaseD is 3'CCA5'
C) RNAseD acts as an endonuclease
D) More than one of the answers
E) coli?

F) A) and B)
G) A) and C)

Correct Answer

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A

What basal transcription factor is a helicase?


A) TFIID
B) TFIIH
C) TFIIF
D) TFIIB

E) C) and D)
F) A) and C)

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The Prinbow box contains a CACAAC consensus sequence located in the -100 region of the promoter.

A) True
B) False

Correct Answer

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Most eukaryotic genes are collinear.


A) True
B) False
C) It is unknown at this time

D) A) and C)
E) All of the above

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Basal transcription in eukaryotes involves the action of only the core promoter.

A) True
B) False

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What would be the effect on in vitro transcription if you replaced the entire TFIID protein with the single subunit TBP?


A) Transcription could not be terminated
B) The initiation complex could not form
C) RNA polymerase II would never be recruited to carry out elongation
D) There would be no effect

E) All of the above
F) B) and C)

Correct Answer

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Which of the following is not an example of a eukaryotic regulatory element?


A) Enhancers
B) Silencers
C) Core promoters
D) Cis-acting elements
E) All of the answers are examples

F) A) and B)
G) None of the above

Correct Answer

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E

The mRNA is read in groups of nucleotides called quadrons.

A) True
B) False

Correct Answer

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What enables the splicing of group I and II introns?


A) Spliceosomes
B) Ribozymes
C) snRNA
D) Poly-A tail

E) A) and D)
F) B) and C)

Correct Answer

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Following transcription, the RNA has a complementary sequence of which of the following?


A) Regulatory sequences
B) Termination sequences
C) The coding strand of DNA
D) The template strand of DNA
E) None of the answers are correct

F) A) and C)
G) A) and E)

Correct Answer

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