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List three enzymatic dyads.

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The most commonly observed enz...

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How does an enzyme attain catalytic perfection?

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An enzyme achieves catalytic p...

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Which of the following amino acids is capable of acting as a general acid or general base at physiological pH?


A) Glycine
B) Histidine
C) Tyrosine
D) Tryptophan
E) Proline

F) B) and C)
G) None of the above

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The synthesis of enzymes in response to changing metabolic needs is referred to as __________.


A) enzyme induction
B) allosteric regulation
C) negative feedback
D) zymogen activation
E) cooperative binding

F) None of the above
G) D) and E)

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What is meant by the term turnover number?

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Turnover number is the number ...

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Which of the following is a coenzyme?


A) NADP+
B) Zn++
C) Cu++
D) Insulin
E) Oxytocin

F) B) and E)
G) None of the above

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What is a reaction intermediate?

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A reaction intermediate is a s...

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Enzymes act by __________.


A) decreasing the energy of activation of a reaction
B) increasing the energy of activation of a reaction
C) raising the temperature of a reaction
D) providing a surface to favorably orient the reactants
E) Both A and D are correct.

F) A) and E)
G) None of the above

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Describe negative feedback inhibition.

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Negative feedback inhibition i...

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Explain why enzyme kinetics measurement are made at the start of a reaction.

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At the start of a reaction, the concentr...

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An enzyme is a large complex molecule much of which is seemingly uninvolved in the enzyme's catalytic function. Would it be possible to eliminate excess polypeptide segments and still retain activity?

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An enzyme's large size is required for c...

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Which of the following is not a property of enzymes?


A) Capable of being regulated
B) Reaction rates high in comparison to uncatalyzed reaction
C) Highly specific
D) Side products of reactions are rare.
E) All of the above are correct.

F) A) and B)
G) C) and D)

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What is meant by the term activation energy?

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Activation energy is...

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In competitive inhibition, increasing the concentration of substrate __________


A) decreases the overall rate of the reaction
B) increases the overall rate of the reaction
C) is without effect
D) the observed effect depends on the inhibitor
E) competitive inhibitors do not affect the rate of the reaction

F) B) and E)
G) A) and B)

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Freeze drying is a useful technique for isolating enzymes. If the pH is not carefully controlled, however, the enzyme frequently becomes denatured. Explain.

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When ice forms, any solutes remaining in...

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Enzyme studies are best carried out in __________.


A) dilute aqueous solution
B) highly concentrated solutions of the enzyme
C) highly concentrated solutions of the substrate
D) the presence of an inert crowding agent
E) the presence of a membrane

F) C) and E)
G) B) and E)

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Is catalytic perfection a function of concentration?

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Catalytic perfection occurs when an enzy...

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NADPH and NADH are coenzymes found in which class of enzymes?


A) Dehydrogenases
B) Ligases
C) Hydrolases
D) Transferases
E) Both C and D are correct.

F) B) and E)
G) A) and E)

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Alcohol dehydrogenase is an example of which of the following classes of enzymes?


A) Oxidoreductases
B) Transferase
C) Hydrolase
D) Lyase
E) Isomerase

F) All of the above
G) D) and E)

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An enzyme without it cofactor is called __________.


A) coenzyme
B) apoenzyme
C) holoenzyme
D) noncatalytic
E) isoenzyme

F) A) and E)
G) B) and E)

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