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How many genes constitute the human genome?


A) More than 1 million
B) 500,000
C) About 25,000
D) 46

E) B) and C)
F) A) and D)

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Which is correct about cDNA?


A) It represents the gene without the introns.
B) Reverse transcriptase is used on the primary transcript.
C) Bacteria possess reverse transcriptase.
D) It is normally found in the nucleus of eukaryotic cells.
E) It is found in the nucleoid of bacteria.

F) A) and C)
G) B) and C)

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A.Argue in support of using adult tissue stem cells rather than embryonic stem cells. B.Argue against using adult stem cells rather than embryonic stem cells.

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Scientists in Scotland figured out a novel method to clone cells successfully.It involved:


A) using an egg and a nucleus that were at the same stage of the cell cycle
B) using an egg that was in cytokinesis and a nucleus that was in S stage of the cell cycle
C) using an egg that was in the S stage of the cell cycle and a nucleus that just passed through telophase
D) using an egg that was in the S stage of the cell cycle and a nucleus that had just entered prophase

E) None of the above
F) B) and C)

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How do you feel about cloning? Explain your position in the light of science.

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The percentage of the human genome made up of structural DNA is _________.

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As development commences in a fertilized human egg,a small ball of cells that forms after about five days is called a _________.

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Adult stem cells are just as totipotent as embryonic stem cells.

A) True
B) False

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False

Removing genes from molecules of DNA requires the use of special:


A) digestive enzymes
B) restriction enzymes
C) enzymes from peroxisomes
D) microscopic scalpels

E) A) and B)
F) A) and C)

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If only about 1% of the human genome is devoted to protein-encoded genes,what is the majority of the rest?


A) Introns.
B) Duplicated sequences.
C) Repeated sequences.
D) Structural DNA.
E) Transposable elements.

F) B) and C)
G) None of the above

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A _______ is a tiny circle of bacterial DNA that is capable of replicating outside of the main bacterial chromosome.

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The genes encoding rRNA are examples of __________________ of genes.

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Which was the genome to be fully sequenced?


A) Oryza sativa (rice)
B) The bacterial virus ΦX174
C) Lily
D) Maple tree

E) C) and D)
F) A) and B)

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Which of the following problems exist when performing human gene therapy?


A) The vector used to carry the gene may elicit a strong immune response,leading to rejection of the added gene.
B) The virus vector may cause a lethal immune reaction itself in rare cases.
C) The virus vector may insert its DNA into the middle of a gene,which could lead to cancer.
D) All of the choices are correct.

E) A) and B)
F) A) and C)

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Ian Wilmut successfully cloned Dolly the sheep from a fully differentiated sheep cell.This type of cloning is known as:


A) reproductive cloning
B) therapeutic cloning
C) stem cell cloning
D) gene therapy cloning

E) A) and B)
F) A) and C)

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When the method is perfected,it will be possible to transfer "healthy" genes into cystic fibrosis patients via aerosol inhalants to "cure" this genetic disease.Is this type of gene transfer an actual cure or is it a treatment? In other words,could this person still pass along cystic fibrosis genes?

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Unless it is possible to correct the genetic defect in the person's gametes,they will still be able to pass on the genes for cystic fibrosis.However,the inhalant treatment is an important milestone in the treatment of this disease,especially if it can put a halt to the lung damage so characteristic of the cystic fibrosis patient.The life expectancy for these patients is short otherwise.

Whole blocks of genes that have been copied from one chromosome to another are referred to as:


A) multigene families
B) segmental duplications
C) tandem clusters
D) codons

E) None of the above
F) A) and B)

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One disease in which gene therapy was used,but not successfully yet is:


A) the common cold
B) cystic fibrosis
C) tuberculosis
D) anthrax

E) A) and B)
F) All of the above

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The name of the virus that causes common colds and has been used as a vector of genes is _______.

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adenovirus

In animal cloning,DNA donated from an adult stem cell:


A) has to be inserted into the egg cell before the egg cell's nucleus is removed
B) has to be reprogrammed for the gene expression needed for early development
C) has to be from another species
D) has to be obtained from an embryonic stem cell

E) All of the above
F) C) and D)

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