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Exam 6: Lipids, Membranes, and the First Cells

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Question 1

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For the following question(s) , match the labeled component of the cell membrane in the figure with its description. -In what way do the membranes of a eukaryotic cell vary?

A) Phospholipids are found only in certain membranes.
B) Certain proteins are unique to each membrane.
C) Only certain membranes of the cell are selectively permeable.
D) Only certain membranes are constructed from amphipathic molecules.
E) Some membranes have hydrophobic surfaces exposed to the cytoplasm, while others have hydrophilic surfaces facing the cytoplasm.

F) A) and B)
G) All of the above

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B

Question 2

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Which of the following allows water to move much faster across cell membranes?

A) the hydrophobic interior of a cell membrane
B) the sodium-potassium pump
C) ATP
D) peripheral proteins
E) aquaporins

F) C) and D)
G) A) and C)

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Question 3

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Which of the following crosses lipid bilayers the slowest?

In some cells, there are many ion electrochemical gradients across the plasma membrane even though there are usually only one or two proton pumps present in the membrane. The gradients of the other ions are most likely accounted for by ________.

The text states that ribonucleotides can diffuse through some types of liposomes. It is likely that the lipids present early in chemical evolution had short chains. Would liposomes formed from these types of lipids be more or less permeable to ribonucleotides than if early cells formed from long-chained lipids?

You make a phospholipid bilayer with short, saturated hydrocarbon tails. You measure the permeability of this membrane to oxygen. You are going to change the length of the hydrocarbon tails and remeasure membrane permeability, but first your boss asks you to graph the data you expect if there is no effect of hydrocarbon tail length on membrane permeability (your null hypothesis) . Which of the accompanying graphs best represents the data you expect if your null hypothesis is correct?

Ions diffuse across membranes through specific ion channels down ________.

Use the following information when answering the corresponding question(s) . Rhodopsins are light-sensitive molecules composed of a protein (opsin) and retinal (derivative of vitamin A) . Opsin is a membrane protein with several α-helical segments that loop back and forth through the plasma membrane. There are two classes of rhodopsins. According to Oded Beje, one class has relatively slow dynamics (a photocycle of approximately 0.5 second) and is well suited for light detection. The second class has faster dynamics (a photocycle of approximately 0.02 seconds) and is well suited for chemiosmosis: pumping of protons or chloride ions across cell membranes. Oded Beje was the first, in September 2000, to report on a rhodopsin (proteorhodopsin) found in the domain Bacteria. [SOURCE: O. Beje et al., Science 289 (2000) : 1902.] -When biological membranes are frozen and then fractured, they tend to break along the middle of the bilayer. The best explanation for this is that ________.

Which of the following is the best explanation for why cholesterol decreases the permeability of biological membranes?

If you mechanically shook a mixture of phospholipids and water, what would you expect to see when you observe the solution using an electron microscope?

Which of the following membrane activities requires energy from ATP?

The solutions in the arms of a U-tube are separated at the bottom of the tube by a selectively permeable membrane. The membrane is permeable to sodium chloride but not to glucose. Side A is filled with a solution of 0.4 M glucose and 0.5 M sodium chloride (NaCl) , and side B is filled with a solution containing 0.8 M glucose and 0.4 M sodium chloride. Initially, the volume in both arms is the same. -Refer to the figure. If you examine side A after three days, you should find ________.

Use the following information when answering the corresponding question(s) . Rhodopsins are light-sensitive molecules composed of a protein (opsin) and retinal (derivative of vitamin A) . Opsin is a membrane protein with several α-helical segments that loop back and forth through the plasma membrane. There are two classes of rhodopsins. According to Oded Beje, one class has relatively slow dynamics (a photocycle of approximately 0.5 second) and is well suited for light detection. The second class has faster dynamics (a photocycle of approximately 0.02 seconds) and is well suited for chemiosmosis: pumping of protons or chloride ions across cell membranes. Oded Beje was the first, in September 2000, to report on a rhodopsin (proteorhodopsin) found in the domain Bacteria. [SOURCE: O. Beje et al., Science 289 (2000) : 1902.] -Refer to the paragraph on rhodopsins. Which of the following best describes this particular protein?

Steroid hormones are large communication molecules that are modified cholesterol molecules. How do you think they enter a cell?

Use the following information when answering the corresponding question(s) . Rhodopsins are light-sensitive molecules composed of a protein (opsin) and retinal (derivative of vitamin A) . Opsin is a membrane protein with several α-helical segments that loop back and forth through the plasma membrane. There are two classes of rhodopsins. According to Oded Beje, one class has relatively slow dynamics (a photocycle of approximately 0.5 second) and is well suited for light detection. The second class has faster dynamics (a photocycle of approximately 0.02 seconds) and is well suited for chemiosmosis: pumping of protons or chloride ions across cell membranes. Oded Beje was the first, in September 2000, to report on a rhodopsin (proteorhodopsin) found in the domain Bacteria. [SOURCE: O. Beje et al., Science 289 (2000) : 1902.] -When a membrane is freeze-fractured, the bilayer splits down the middle between the two layers of phospholipids. In an electron micrograph of a freeze-fractured membrane, the bumps seen on the fractured surface of the membrane are ________.

Cooking oil and gasoline (a hydrocarbon) are NOT amphipathic molecules because they ________.

Lipids ________.

Glucose diffuses slowly through artificial phospholipid bilayers. The cells lining the small intestine, however, rapidly move large quantities of glucose from the glucose-rich food into their glucose-poor cytoplasm. Using this information, which transport mechanism is most probably functioning in the intestinal cells?

Which of the following crosses lipid bilayers the fastest?

In an experiment involving planar bilayers, a solution of table salt (sodium and chloride ions in water) is added on the left side of the membrane while pure water is added on the right side. After 30 minutes, the researchers test for the presence of ions on each side of the membrane. The right side tests negative for ions. What can you conclude?