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Exam 17: Gene Expression: From Gene to Protein

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Question 31

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E = exon and I = intron 5′-UTR E1 I1 E2 I2 E3 I3 E4 UTR-3′ Which components of the previous molecule will also be found in mRNA in the cytosol?

A) 5′-UTR I1 I2 I3 UTR-3′
B) 5′-E1 E2 E3 E4-3′
C) 5′-UTR E1 E2 E3 E4 UTR-3′
D) 5′-E1 I1 E2 I2 E3 I3 E4-3′

E) B) and D)
F) B) and C)

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Question 32

Multiple Choice

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The figure shows a simple metabolic pathway. -If A, B, and C are all required for growth, a strain that is mutant for the gene-encoding enzyme A would be able to grow on medium supplemented with which of the following nutrient(s) ?

A) nutrient A only
B) either nutrient B or C
C) nutrient C only
D) nutrients A and C

E) A) and B)
F) All of the above

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Question 33

Multiple Choice

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Which component is not directly involved in translation?

Which of the following molecules are required for the process of translation?

Which of the following processes occurs in eukaryotic gene expression?

Which of the following DNA mutations is most likely to damage the protein it specifies?

What is the function of the release factor during translation in eukaryotes?

Which of the following mutations would be most likely to have a harmful effect on an organism?

A part of an mRNA molecule with the following sequence is being read by a ribosome: 5′-CCG-ACG-3′ (mRNA) . The following charged transfer RNA molecules (with their anticodons shown in the 3′ to 5′ direction) are available. Two of them can correctly match the mRNA so that a dipeptide can form. $\begin{array} { | c | c | } \hline \text { tRNA Anticodon } & \text { Amino Acid } \\\hline \text { GGC } & \text { Proline } \\\text { CGU } & \text { Alanine } \\\text { UGC } & \text { Threonine } \\\text { CCG } & \text { Glycine } \\\text { ACG } & \text { Cysteine } \\\text { CGG } & \text { Alanine } \\\hline\end{array}$ - Which of the following dipeptides will form from this mRNA?

In the structural organisation of many eukaryotic genes, individual exons may be related to which of the following?

Which of the following statements correctly describes the effect a nonsense mutation would have on a gene?

- Which of the following sequences of nucleotides are possible in the template strand of DNA that would code for the polypeptide sequence Phe-Leu-Ile-Val?

Which of the following statements supports the one gene-one enzyme hypothesis?

Which of the following processes occurs during transcription?

Which one of the following structures, if missing, would usually prevent translation from starting?

How does the primary transcript in the nucleus of a eukaryotic cell compare to the functional mRNA?

Codons are three-base sequences in mRNA that specify the addition of a single amino acid to the growing protein chain during translation. How do eukaryotic codons and prokaryotic codons compare?

- Refer to the figure. Which of the triplets below is a possible anticodon for a tRNA that transports proline to a ribosome?

Which of the following types of mutation, resulting in an error in the mRNA just after the AUG start of translation, is likely to have the most serious effect on the polypeptide product?

Use the figure to identify a 5′ → 3′ sequence of nucleotides in the DNA template strand for an mRNA coding for the polypeptide sequence Phe-Pro-Lys.