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By creating gene knockouts, researchers can study how the loss of normal gene function affects the organism

A) True
B) False

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A transgenic organism has recombinant DNA integrated into its genome.

A) True
B) False

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Which of the following can be used as a vector for gene therapy?


A) Liposomes
B) Parvoviruses
C) Adenoviruses
D) Retroviruses
E) All of the answers are correct

F) A) and C)
G) B) and E)

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Bone marrow transplants typically use what type of cells?


A) Embryonic stem cells
B) Embryonic germ cells
C) Embryonic carcinoma cells
D) Hematopoietic stem cells

E) A) and C)
F) None of the above

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Which of the following mechanisms uses high-velocity microprojectiles, coated with DNA, to produce a transgenic plant?


A) A. tumefaciens
B) Electroporation
C) Microinjection
D) Biolistic gene transfer

E) A) and B)
F) None of the above

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In the production of transgenic plants, what selectable marker is most commonly used to indicate that a cell has taken up the T DNA that includes the cloned gene?


A) Ampicillin
B) Neomycin
C) Streptomycin
D) Kanamycin

E) A) and C)
F) A) and B)

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When researchers produced a gene knockin by introducing the mutant human β-globin gene into mice, the resulting mice showed very severe symptoms of the disease.

A) True
B) False

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A major disadvantage with current gene therapy techniques using viral vectors is the possibility of an immune response against the virus.

A) True
B) False

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Which of the following organisms is used by molecular biologists to produce transgenic plants?


A) E. coli
B) Plasmid pSC101
C) Bacteriophage l
D) Agrobacterium tumefaciens

E) A) and D)
F) None of the above

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Identical twins are an example of gene cloning.

A) True
B) False

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All stem cells have the potential to differentiate

A) True
B) False

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The first hormone to be made by recombinant bacteria was ________.


A) Insulin
B) Glucagon
C) Somatostatin
D) Testosterone

E) All of the above
F) A) and D)

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Hematopoietic stem cells are ________.


A) Pluripotent
B) Totipotent
C) Unipotent
D) Multipotent

E) B) and C)
F) B) and D)

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The primary interest of genetic research into stem cells is the desire to be able to clone a human being.

A) True
B) False

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An organism that can be regenerated by somatic cells is called multipotent.

A) True
B) False

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In the treatment of ADA deficiency and SCID, which cell type was targeted for gene therapy?


A) Fibroblasts
B) Embryonic stem cells
C) Hematopoietic stem cells
D) Lymphocytes
E) Neutrophils

F) B) and C)
G) A) and E)

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Which of the following is not an advantage of a transgenic plant?


A) Widely accepted by the public
B) Increased resistance to disease and pests
C) Adaptability to harsh environments
D) Improved shelf life

E) A) and B)
F) A) and C)

Correct Answer

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